N-(1-Thioxoethyl)glycine is primarily used in the field of organic synthesis and biochemistry. It serves as a key intermediate in the preparation of various thioamide compounds, which are valuable in the development of pharmaceuticals and agrochemicals. This chemical is also utilized in the study of enzyme inhibition, particularly in research focused on understanding the mechanisms of thiol-dependent enzymes. Additionally, it finds application in the synthesis of peptides and peptidomimetics, where it contributes to the introduction of thioamide bonds, enhancing the stability and bioactivity of the resulting molecules. Its role in modifying protein structures and functions makes it a significant compound in biochemical research and drug design.