Reverse phase silica gel plates with fluorescence indicator are widely used in thin-layer chromatography (TLC) for separating and analyzing chemical compounds, especially non-polar substances. They serve as a non-polar stationary phase, enabling the separation of mixtures based on hydrophobicity, polarity, and molecular interactions. These plates are essential in laboratories for identifying components in pharmaceuticals, natural products, and chemical reactions. They are also employed in quality control processes to ensure the purity of substances. Additionally, reverse phase silica gel plates are used in forensic analysis to detect and identify unknown substances in samples. The fluorescence indicator allows for UV visualization of compounds. Their versatility and efficiency make them a fundamental tool in analytical chemistry.