EW-7197 Hydrochloride is used primarily in biomedical research as a selective inhibitor of TGF-β receptor type I (ALK5), targeting specific proteins involved in fibrosis and cancer progression. Its application is significant in studying cellular signaling pathways, particularly those related to TGF-β, which plays a role in tissue scarring and tumor growth. Researchers utilize it to explore potential therapeutic strategies for conditions like pulmonary fibrosis, liver fibrosis, chronic kidney disease, and certain cancers. It is also employed in preclinical studies to evaluate its efficacy in reducing fibrotic responses and inhibiting metastasis, especially in TGF-β overexpressing cancers.