This chemical is primarily utilized in the field of bioconjugation and chemical biology, particularly in click chemistry reactions. Its tetrazine moiety enables efficient and selective reactions with strained alkenes or alkynes, making it valuable for labeling biomolecules such as proteins, nucleic acids, and cells. It is often employed in the development of targeted drug delivery systems, where it facilitates the attachment of therapeutic agents to specific biological targets. Additionally, it is used in bioimaging applications to track and visualize cellular processes in real-time. Its stability and reactivity under mild conditions make it a versatile tool in research and pharmaceutical development.