3,6-Diphenyl-1,2,4,5-tetrazine is widely used in bioorthogonal chemistry due to its high reactivity in inverse electron demand Diels-Alder (IEDDA) reactions. It is particularly valuable in labeling and imaging applications, where it selectively reacts with strained alkenes or alkynes in biological systems without interfering with native biochemical processes. This property makes it a key component in live-cell imaging, enabling researchers to track and visualize biomolecules in real-time. Additionally, it is employed in the development of bioconjugation strategies for attaching functional groups to proteins, nucleic acids, or other biomolecules. Its use extends to materials science, where it serves as a crosslinking agent in the synthesis of advanced polymers and nanomaterials.