This chemical is primarily used in bioconjugation and click chemistry applications, particularly in the field of bioorthogonal chemistry. Its tetrazine group enables rapid and selective reactions with strained alkenes or alkynes, making it valuable for labeling biomolecules such as proteins, nucleic acids, and cells. It is widely employed in live-cell imaging, where its fast reaction kinetics allow for real-time tracking of biological processes. Additionally, it is utilized in drug delivery systems to attach targeting moieties or therapeutic agents to carriers. Its stability and efficiency in complex biological environments make it a key tool in developing diagnostic probes and therapeutic conjugates.