This chemical is primarily utilized in bioorthogonal chemistry, particularly in the field of bioconjugation and labeling of biomolecules. Its tetrazine group enables rapid and selective reactions with dienophiles, such as trans-cyclooctenes or norbornenes, through the inverse electron-demand Diels-Alder (IEDDA) reaction. This makes it highly effective for labeling proteins, nucleic acids, or other biomolecules in live cells or complex biological systems without interfering with native biochemical processes. The polyether chain enhances water solubility, making it suitable for use in aqueous environments, while the alkyne group allows for further functionalization via click chemistry. Its applications include imaging, drug delivery, and the study of molecular interactions in real-time.