This chemical is primarily utilized in bioconjugation and click chemistry applications, particularly in the labeling and modification of biomolecules. Its tetrazine moiety enables efficient and rapid reactions with strained alkenes or alkynes, making it valuable for bioorthogonal labeling in live cells and tissues. This allows for precise tracking and imaging of proteins, nucleic acids, or other biomolecules in complex biological systems. Additionally, the biotin group in its structure facilitates strong binding to avidin or streptavidin, enabling its use in affinity purification, detection, and immobilization techniques. This dual functionality makes it a versatile tool in proteomics, diagnostics, and targeted drug delivery research.