This compound is widely utilized in bioconjugation chemistry, particularly for labeling biomolecules such as proteins, peptides, and antibodies. Its tetrazine moiety enables rapid and selective reactions with trans-cyclooctene (TCO) via inverse electron-demand Diels-Alder (IEDDA) cycloaddition, making it valuable for bioorthogonal labeling in live cells and complex biological systems. The PEG linker enhances solubility and reduces steric hindrance, facilitating efficient conjugation in aqueous environments. It is also employed in the development of targeted drug delivery systems, where it helps attach therapeutic agents to specific biomolecules. Additionally, it finds applications in imaging and diagnostics, enabling the precise tracking of molecular interactions in real-time studies.