Fumarate hydratase-IN-1 is primarily used in biochemical research to study the role of fumarate hydratase (FH) in cellular metabolism. It serves as an inhibitor of FH, an enzyme involved in the tricarboxylic acid (TCA) cycle, which is crucial for energy production in cells. By inhibiting FH, researchers can investigate the enzyme's impact on metabolic pathways, particularly in conditions like cancer, where FH mutations are linked to tumorigenesis. This compound is also utilized to explore the relationship between FH dysfunction and the accumulation of fumarate, which can lead to epigenetic changes and altered cellular signaling. Its application extends to understanding metabolic reprogramming in diseases and developing potential therapeutic strategies targeting FH-related pathways.