CCT031374 hydrobromide is primarily utilized in research settings as a potent and selective inhibitor of the Tankyrase enzyme, which plays a critical role in the Wnt/β-catenin signaling pathway. This pathway is often dysregulated in various cancers, making CCT031374 hydrobromide a valuable tool for studying cancer biology and potential therapeutic strategies. By inhibiting Tankyrase, it helps researchers understand the mechanisms of tumor growth and metastasis, particularly in colorectal cancer and other malignancies driven by Wnt signaling. Additionally, it is used in drug discovery programs to develop novel anticancer agents targeting this pathway. Its application extends to studying cellular processes such as telomere maintenance, cell proliferation, and tissue regeneration, providing insights into both normal and pathological conditions.