WIKI4 is a small molecule inhibitor primarily used in medicinal chemistry and drug development. It selectively targets tankyrase 1 and 2 (TNKS1/2), enzymes involved in the Wnt/β-catenin signaling pathway, which is dysregulated in various cancers, fibrosis, and inflammatory diseases. Researchers use WIKI4 to investigate cellular signaling mechanisms, stabilize Axin to promote β-catenin degradation, and explore therapeutic interventions. In preclinical studies, it evaluates the inhibition of tumor growth, metastasis, and fibrotic processes. WIKI4 is also employed in biochemical and high-throughput assays to assess tankyrase activity and the downstream effects on gene expression and cell proliferation. Its specificity makes it a key tool in advancing oncology, fibrosis research, and targeted drug discovery.