JK184 is primarily utilized in biological research, particularly in the study of cellular signaling pathways. It is known for its role as a potent inhibitor of the Wnt/β-catenin signaling pathway, which is crucial in understanding various cellular processes, including cell proliferation, differentiation, and apoptosis. By inhibiting this pathway, JK184 helps researchers investigate the mechanisms underlying diseases such as cancer, where Wnt signaling is often dysregulated. Additionally, JK184 is used in developmental biology studies to explore the role of Wnt signaling in tissue regeneration and embryonic development. Its application extends to drug discovery, where it serves as a tool compound to identify potential therapeutic targets for conditions linked to aberrant Wnt signaling.