Isomaltotetraose is widely used in the food and beverage industry as a functional oligosaccharide. It serves as a prebiotic, promoting the growth of beneficial gut bacteria, which supports digestive health. Its mild sweetness and low caloric content make it a suitable ingredient in low-sugar and diet-friendly products. Additionally, it is utilized in the production of functional foods and supplements aimed at enhancing gut microbiota balance. Its stability under various processing conditions also makes it ideal for incorporation into baked goods, dairy products, and beverages. Furthermore, it is explored in pharmaceutical applications for its potential to improve gut health and immune function.