This synthetic glycolipid, Lac β(1-4)Lac-β-C10, is primarily utilized in glycobiology and biochemistry research. It functions as a substrate for investigating enzymatic activities of glycosyltransferases and glycosidases, which play key roles in carbohydrate metabolism and cell signaling pathways. Furthermore, it serves as a building block in the synthesis of complex glycoconjugates for vaccine development and therapeutic applications targeting carbohydrate-related diseases.