Trithioacetone is primarily used in organic synthesis as a sulfur-containing building block. Its reactive thio-carbonyl groups make it valuable for constructing heterocyclic compounds, particularly those involving sulfur atoms in the ring structure. It serves as a precursor in the preparation of various organosulfur compounds, which find applications in pharmaceuticals, agrochemicals, and materials science. Due to its ability to participate in cycloaddition and condensation reactions, it is employed in research settings to develop new sulfur-rich molecules with potential biological activity. Additionally, trithioacetone has been explored in the synthesis of ligands for metal coordination chemistry, where sulfur donor atoms can stabilize metal centers in catalytic systems. Its use remains mostly confined to laboratory-scale synthesis due to its instability and strong odor.