Used as a fluorescent labeling agent in biochemical and analytical applications. Its pyrene moiety provides strong fluorescence with high quantum yield, making it suitable for detecting and tracking biomolecules such as proteins and nucleic acids. The compound reacts with primary amines on target molecules to form stable amide bonds, allowing for covalent attachment of the fluorophore. Commonly employed in fluorescence spectroscopy, imaging, and immunoassays where sensitive detection is required. Also utilized in the development of fluorescent probes and sensors due to its environmental sensitivity and long fluorescence lifetime.