16-Dehydropregnenolone is primarily used as an intermediate in the synthesis of various steroid hormones. It plays a crucial role in the production of corticosteroids, which are essential for treating inflammation, autoimmune diseases, and allergies. Additionally, it is utilized in the development of sex hormones, including testosterone and estrogen, which are vital for managing hormonal imbalances and reproductive health. Its application extends to the pharmaceutical industry, where it serves as a key starting material for creating therapeutic agents targeting endocrine disorders. Researchers also employ it in studies focused on understanding steroid metabolism and developing novel drug compounds.