Estradiene dione-3-keta is primarily utilized in the field of steroid chemistry and endocrinology. It serves as a key intermediate in the synthesis of various steroid hormones, including estrogens and androgens. Researchers and pharmaceutical companies employ this compound to develop hormone-based therapies, particularly for conditions related to hormonal imbalances. Additionally, it is used in the production of contraceptive drugs and treatments for menopausal symptoms. In laboratory settings, it is a valuable reagent for studying steroid metabolism and the biochemical pathways involved in hormone regulation. Its role in synthesizing biologically active steroids makes it essential for both therapeutic and research applications.