Thiocholesterol is primarily used in the field of biochemistry and material science. It serves as a key component in the formation of self-assembled monolayers (SAMs) on gold surfaces, which are essential for studying molecular interactions and surface modifications. These SAMs are widely applied in biosensors to enhance sensitivity and specificity in detecting biological molecules. Additionally, thiocholesterol is utilized in the development of drug delivery systems, where it helps stabilize lipid-based carriers like liposomes, improving their efficiency in targeting and releasing therapeutic agents. Its role in modifying surface properties also makes it valuable in creating biocompatible coatings for medical devices.