Hyodeoxycholic acid is primarily used in the pharmaceutical industry for the synthesis of ursodeoxycholic acid, a medication that helps dissolve gallstones and treat liver diseases. It also serves as a key intermediate in the production of various bile acid derivatives, which are essential for research and development in hepatology and gastroenterology. Additionally, it has potential applications in the study of cholesterol metabolism and as a reference standard in analytical chemistry for bile acid analysis. Its role in modulating lipid profiles makes it valuable in exploring therapeutic strategies for metabolic disorders.