This compound is a key intermediate in the synthesis of steroidal neuromuscular blocking agents, such as pancuronium bromide, a non-depolarizing muscle relaxant used during anesthesia to facilitate endotracheal intubation and provide skeletal muscle relaxation during surgery. Its specific structure, featuring 2-(4-morpholinyl), 16-(1-pyrrolidinyl) substituents, 3-hydroxy, and 17-acetoxy groups on the androstane skeleton, enables selective protection and quaternization to form active bis-quaternary ammonium salts that competitively antagonize nicotinic acetylcholine receptors at the neuromuscular junction. It is utilized in medicinal chemistry and pharmacology research for developing improved paralytic agents with enhanced potency, duration of action, and safety profiles.