15-oxospiramilactone is primarily utilized in the field of medicinal chemistry and pharmaceutical research. It serves as a key intermediate in the synthesis of various steroidal compounds, which are often investigated for their potential therapeutic effects. One of its significant applications is in the development of anti-inflammatory and anti-cancer agents, where its structure is modified to enhance biological activity and selectivity. Additionally, it is explored in the study of enzyme inhibition, particularly targeting enzymes involved in steroid metabolism, which can be relevant for treating hormone-related disorders. Its role in drug discovery and development underscores its importance in creating novel treatments for complex diseases.