OH-Chol is widely used in the field of biochemistry and medical research as a key intermediate in the synthesis of bile acids and steroids. It plays a crucial role in studying cholesterol metabolism and its impact on cardiovascular health. Researchers utilize OH-Chol to investigate the mechanisms of lipid regulation and to develop potential therapies for conditions like hypercholesterolemia. Additionally, it is employed in the production of pharmaceutical compounds aimed at treating liver and gallbladder disorders. Its application extends to the development of diagnostic tools for assessing cholesterol-related diseases, making it a valuable component in both therapeutic and diagnostic advancements.