Glycodeoxycholic acid monohydrate (GDCA) is primarily used in biochemical research to study bile acid metabolism and its role in digestive processes. It serves as a key component in experiments exploring the interaction between bile acids and cellular receptors, particularly in the liver and intestines. GDCA is also utilized in the development of therapeutic agents targeting bile acid-related disorders, such as cholestasis and gallstone formation. Additionally, it is employed in in vitro studies to investigate its effects on cell signaling pathways and membrane transport mechanisms. In pharmaceutical formulations, GDCA can act as a solubilizing agent for poorly water-soluble drugs, enhancing their bioavailability. Its application extends to the study of gut microbiota, where it helps understand the impact of bile acids on microbial composition and function.