5β-Androsterone is primarily used in research settings to study steroid metabolism and its effects on the human body. It serves as a key biomarker in clinical diagnostics to assess the activity of certain enzymes involved in steroid hormone pathways, particularly in conditions related to adrenal and gonadal function. Additionally, it is investigated for its potential role in understanding androgen-related disorders and metabolic processes. In the field of endocrinology, it helps in evaluating hormonal imbalances and their impact on health. Its applications also extend to forensic science, where it can be used to detect steroid use or abuse.