This compound is primarily utilized in research and analytical chemistry as a stable isotope-labeled internal standard. It is particularly valuable in mass spectrometry-based studies for the quantification of cholesterol and its metabolites in biological samples. By serving as a reference compound, it enhances the accuracy and precision of measurements, enabling researchers to study lipid metabolism, cholesterol biosynthesis, and related pathways in greater detail. Its application is critical in fields such as biochemistry, pharmacology, and clinical diagnostics, where understanding cholesterol dynamics is essential for investigating diseases like atherosclerosis, metabolic disorders, and cardiovascular conditions.