Estrone-[2,3,4-13C3] is primarily used as a stable isotope-labeled compound in research and analytical applications. It serves as an internal standard in mass spectrometry for the accurate quantification of estrone in biological samples, such as blood, urine, or tissue. This labeled compound helps researchers distinguish between endogenous estrone and external sources, improving the precision of hormone level measurements. It is also employed in metabolic studies to trace the pathways and transformations of estrone in the body, aiding in the understanding of estrogen metabolism and its role in health and disease. Additionally, it is utilized in the development and validation of diagnostic assays for hormonal disorders.