Methyl hyodeoxycholate is primarily utilized in the pharmaceutical and research sectors due to its role as a bile acid derivative. It is often employed in studies focusing on bile acid metabolism and its impact on lipid digestion and absorption. Researchers use it to investigate the mechanisms of bile acids in cholesterol regulation and their potential therapeutic applications in metabolic disorders. Additionally, it serves as a reference standard in analytical chemistry for the identification and quantification of bile acids in biological samples. Its applications extend to the development of drugs targeting liver and gastrointestinal diseases, where bile acids play a critical role.