24, 25-Dihydroxy VD3 plays a significant role in calcium and phosphate metabolism, contributing to bone health and mineralization. It is utilized in research to study vitamin D metabolism and its effects on bone formation and resorption. This compound is also explored in clinical studies for its potential therapeutic applications in treating bone disorders, such as osteoporosis, by enhancing bone density and strength. Additionally, it is investigated for its role in regulating immune responses and its potential benefits in autoimmune diseases. Researchers use it to understand the complex pathways of vitamin D and its active metabolites in maintaining overall health.