This compound plays a significant role in the study of vitamin D metabolism, particularly in understanding the pathways and biological effects of vitamin D2. It is often used in research to investigate the regulation of calcium and phosphate levels in the body, which are crucial for bone health. Additionally, it serves as a reference standard in analytical chemistry to identify and quantify vitamin D metabolites in biological samples. Its application extends to exploring potential therapeutic uses in conditions related to vitamin D deficiency or disorders of calcium metabolism.