1alpha, 24, 25-Trihydroxy VD2 is primarily used in medical and pharmaceutical research due to its role in calcium and phosphate metabolism. It is studied for its potential therapeutic effects in treating bone disorders, such as osteoporosis, by promoting bone mineralization and reducing bone resorption. Additionally, it is explored for its immunomodulatory properties, which may benefit autoimmune diseases. In dermatology, it is investigated for treating skin conditions like psoriasis due to its ability to regulate cell proliferation and differentiation. Its applications also extend to cancer research, where it is examined for its potential to inhibit tumor growth and induce cancer cell differentiation.