This bile acid derivative, featuring a 3-oxo-7,12-dihydroxy substitution on the cholane skeleton, serves as a valuable intermediate in organic synthesis and biochemical research. It is employed in studies of steroid and bile acid metabolism, hydroxysteroid dehydrogenase activities, and the development of bile acid analogs for pharmaceutical applications. These include therapeutics for cholestatic liver diseases, gallstones, inflammatory conditions, metabolic disorders, and certain hormone-related pathways via nuclear receptor modulation (e.g., FXR, TGR5).