Used primarily in research to study bile acid metabolism and its role in liver and intestinal health. It serves as a reference standard in analytical techniques like HPLC and mass spectrometry for quantifying bile acids in biological samples. Also applied in investigating the regulation of cholesterol homeostasis and the enterohepatic circulation of bile acids. Its properties make it valuable for exploring the interaction between bile acids and gut microbiota, aiding in understanding metabolic disorders and potential therapeutic targets.