16-Dehydroprogesterone is primarily used in the field of endocrinology and reproductive medicine. It serves as a key intermediate in the synthesis of various steroid hormones, including corticosteroids and sex hormones. In pharmaceutical research, it is utilized to develop progesterone analogs and other steroid-based drugs that regulate menstrual cycles, support pregnancy, and manage hormonal imbalances. Additionally, it plays a role in studying the biochemical pathways of steroidogenesis, aiding in the understanding of hormone-related disorders. Its applications also extend to the development of anti-inflammatory and immunosuppressive medications due to its structural similarity to active steroid compounds.