5β-Dihydroprogesterone is a key metabolite of progesterone produced via 5β-reductase enzyme activity. It is primarily used in biochemical research to investigate steroid hormone metabolism, focusing on the inactivation pathways that regulate progesterone levels in the body, particularly in the liver and reproductive tissues. This compound helps elucidate mechanisms of hormone balance in reproductive and peripheral systems, as it represents a biologically inactive form compared to active steroid derivatives. It is also employed as a reference standard in analytical laboratories for the quantification of progesterone metabolites in biological samples, such as blood and urine.