Used in biochemical research to study cholesterol metabolism and its role in cellular processes. It helps in understanding the mechanisms of cholesterol oxidation and its impact on lipid homeostasis. This compound is also utilized in investigating the signaling pathways related to liver X receptors (LXRs), which are involved in regulating cholesterol, fatty acid, and glucose metabolism. Additionally, it serves as a reference standard in analytical studies to identify and quantify similar sterols in biological samples.