Used primarily in clinical laboratories for the detection of mycobacteria, such as Mycobacterium tuberculosis and Mycobacterium leprae, in patient specimens like sputum, tissue, and bodily fluids. The cold staining method does not require heating, making it safer and easier to perform compared to hot staining techniques. It allows for consistent staining of acid-fast organisms, which retain the primary dye (carbol fuchsin) due to their high mycolic acid content in the cell wall. The kit helps differentiate acid-fast bacteria from non-acid-fast cells under the microscope, supporting rapid diagnosis of tuberculosis and other mycobacterial infections. It is widely used in resource-limited settings due to its simplicity, low cost, and minimal equipment requirements.