Used in histopathology laboratories to detect Helicobacter pylori in gastric tissue samples. The stain helps visualize the bacteria in biopsy specimens by selectively coloring the organisms blue, allowing pathologists to identify their presence and distribution within the gastric mucosa. This aids in diagnosing H. pylori-associated gastritis, peptic ulcers, and assessing risk for gastric cancer. The method is valued for its simplicity, cost-effectiveness, and compatibility with routine tissue staining workflows.