Used in microbiology laboratories to differentiate bacterial species into two large groups—Gram-positive and Gram-negative—based on the chemical and physical properties of their cell walls. The enhanced version improves staining contrast and reliability, making it easier to identify bacteria in clinical samples such as blood, urine, cerebrospinal fluid, sputum, and wound swabs. Commonly applied in diagnosing infections like pneumonia, urinary tract infections, septicemia, meningitis, and other acute conditions. The enhancement reduces false results and supports faster decision-making in patient care, especially in critical and emergency settings, while aiding in appropriate antibiotic selection. Also used in research and quality control in pharmaceutical and food industries to detect bacterial contamination.