YM-53601 free base is primarily utilized in research settings to study its effects on lipid metabolism and cholesterol biosynthesis. It acts as a potent inhibitor of squalene synthase, an enzyme crucial in the cholesterol synthesis pathway. By inhibiting this enzyme, it helps researchers understand the regulation of cholesterol levels and explore potential therapeutic targets for hypercholesterolemia, hyperlipidemia, and related cardiovascular diseases. Additionally, it is used in pharmacological studies to investigate its potential in reducing atherosclerosis and other lipid-related disorders. Preliminary research also suggests it may have a role in inhibiting the growth of certain cancer cells. Its application extends to preclinical studies aimed at developing novel treatments for metabolic diseases. However, studies are still experimental, and further research is needed to confirm efficacy and safety for clinical use.