N-tert-Butyl-α-phenylnitrone is widely used as a spin trap in electron paramagnetic resonance (EPR) spectroscopy to detect and identify free radicals in chemical and biological systems. Its ability to form stable adducts with radicals makes it a valuable tool in studying oxidative stress and related mechanisms in cells and tissues. In biomedical research, it helps investigate the role of free radicals in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. Additionally, it is utilized in material science to analyze radical reactions in polymers and other materials, aiding in the development of more stable and durable products. Its application in antioxidant studies also contributes to the evaluation of potential therapeutic agents.