Used in advanced lipid research and cellular signaling studies due to its role as a bioactive sphingolipid metabolite. It serves as a key reagent in investigating sphingolipid-mediated pathways, particularly those involved in cell proliferation, apoptosis, and stress responses. Commonly employed in in vitro assays to modulate enzyme activity, such as phospholipase D or sphingosine kinase, and helps in understanding membrane microdomain function in lipid rafts. Its ammonium salt form enhances solubility and stability in aqueous-based experimental systems, making it suitable for cell culture and biochemical applications. Also utilized in the development of lipid-based therapeutics and drug delivery models targeting sphingolipid dysregulation in diseases like cancer, neurodegeneration, and inflammatory disorders.