Gb4Ceramide plays a significant role in cellular recognition and signaling processes, particularly in the context of cancer biology. It is overexpressed in certain tumor types, including breast, ovarian, and melanoma cancers, making it a potential target for immunotherapy and diagnostic imaging. Due to its presence on the surface of cancer cells, Gb4Ceramide is being explored as a tumor-associated antigen for vaccine development and antibody-based therapies. Additionally, it contributes to the regulation of cell adhesion and membrane organization, influencing tumor progression and metastasis. Research also suggests involvement in pathogen attachment, as some viruses and bacteria may use this molecule as a receptor for host cell entry, highlighting its relevance in infectious disease studies.