C12 NBD Ceramide (d18:1/12:0) is widely used as a fluorescent lipid probe in biological research to study lipid metabolism, membrane dynamics, and intracellular trafficking. Its fluorescent properties make it an excellent tool for visualizing and tracking ceramide distribution and movement within live cells. Researchers often employ it to investigate processes such as endocytosis, exocytosis, and lipid sorting in various cellular compartments. Additionally, it is utilized in studies focused on understanding the role of ceramides in signaling pathways, apoptosis, and membrane biophysics. Its ability to integrate into lipid bilayers without disrupting membrane integrity makes it a valuable reagent for fluorescence microscopy and flow cytometry experiments.