2,5,8,11-Tetraoxatetradec-13-ene is primarily used as a reactive intermediate in the synthesis of complex organic compounds, especially in polymer production and the manufacture of surfactants. Its structure, featuring multiple ether linkages and a terminal double bond, enables facile reactivity in various chemical processes. It also serves as a starting material in pharmaceutical synthesis for certain bioactive agents. Furthermore, it is utilized in research for developing advanced materials with tailored properties, such as enhanced flexibility and chemical resistance.