ML-226 is primarily used in biochemical research as a selective inhibitor of the enzyme soluble epoxide hydrolase (sEH). This enzyme plays a significant role in the metabolism of epoxyeicosatrienoic acids (EETs), which are involved in various physiological processes, including inflammation and blood pressure regulation. By inhibiting sEH, ML-226 helps increase the levels of EETs, which can have protective effects in cardiovascular and inflammatory diseases. Researchers utilize ML-226 to study the pathways and mechanisms of sEH and its potential therapeutic applications in conditions such as hypertension, pain, and chronic inflammatory disorders. Its application is crucial for advancing understanding in pharmacology and drug development targeting sEH-related pathways.