Used for the detection and quantification of nickel (Ni) bioavailability in soil samples, this ELISA kit enables rapid assessment of nickel that is biologically active and potentially toxic to plants and microorganisms. It supports environmental monitoring by identifying the fraction of nickel that can be taken up by roots or interact with soil biota, offering a more accurate risk assessment than total metal content. Commonly applied in contaminated land studies, agriculture, and remediation projects to evaluate metal mobility and effectiveness of soil treatments. Suitable for field and lab use, providing a high-throughput, cost-effective alternative to traditional analytical methods.