Tenovin-3 is primarily utilized in research settings to study its effects on cellular processes, particularly in the context of cancer biology. It is known to inhibit sirtuin activity, which plays a role in regulating cellular aging and stress responses. This makes it a valuable tool for investigating pathways involved in tumor growth and survival. Researchers also explore its potential in modulating autophagy and apoptosis, providing insights into therapeutic strategies for cancer treatment. Additionally, Tenovin-3 is used in studies focusing on the role of sirtuins in metabolic disorders and neurodegenerative diseases, contributing to the development of novel treatment approaches. Its application extends to chemical biology, where it helps in understanding protein function and interaction networks.