O-(tert-Butyldimethylsilyl)hydroxylamine is primarily used in organic synthesis as a protecting group for hydroxylamine. It is particularly valuable in the preparation of complex molecules where selective protection and deprotection of functional groups are required. The compound is often employed in the synthesis of oximes, which are intermediates in the production of various pharmaceuticals and agrochemicals. Additionally, it finds application in the modification of peptides and other biomolecules, enabling researchers to study their structure and function more effectively. Its stability under a range of reaction conditions makes it a versatile tool in synthetic chemistry.